HuH-7-CAS9 cell line

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  • HuH-7 HuH-7-CAS9-HuH-7
  • HuH-7-CAS9 cell line HuH-7-CAS9-产品图
HuH-7 HuH-7-CAS9-HuH-7
HuH-7-CAS9 cell line HuH-7-CAS9-产品图
HuH-7-CAS9 Copy Product Info
Catalog#： YC-D001-Cas9-H
Size： 1*10^6
Culture method： 90%DMEM+10% FBS
STR Report
Datasheet (Use Instruction)
  • HuH-7 HuH-7-CAS9-HuH-7
  • HuH-7-CAS9 cell line HuH-7-CAS9-产品图
HuH-7-CAS9 Copy Product Info
Catalog#： YC-D001-Cas9-H
Size： 1*10^6
Culture method： 90%DMEM+10% FBS
Datasheet (Use Instruction)

Detailed Product Information

Cell Info

Product category Cas9 Stable Cell Lines Mycoplasma test negative
Cell Morphology Epithelial-like, adherent Culture method 90%DMEM+10% FBS
Passage ratio 1:2~1:3 Species Human
Expression gene Cas9 Resistance gene hygro
Product category
Cas9 Stable Cell Lines
Mycoplasma test
negative
Cell Morphology
Epithelial-like, adherent
Culture method
90%DMEM+10% FBS
Passage ratio
1:2~1:3
Species
Human
Expression gene
Cas9
Resistance gene
hygro
Recombinant Cas9 protein derived from Streptococcus pyogenes is a protein that plays a key role in CRISPR/Cas9 system. It has endonuclease activity; Under the guidance of sgRNA, Cas9 protein can move to the target gene site, cut DNA accurately, and cause DNA double-strand breaks. Ubigene's HuH-7 cell line was constructed on the basis of wild-type HuH-7 cells by lentiviral transduction of the engineered Cas9 protein gene, which can stably express Cas9 protein for a long time. Using this cell, efficient gene knockout, knock-in and point mutation in HuH-7 cells can be achieved by only transfecting sgRNA or co-transfecting sgRNA and donor DNA. It can also be used for the construction of cell knockout library and easily solve the problem of Cas9 transfection difficulty.

Product Validation Data

RT-qPCR
Sample Name Target Name Cт Mean ΔCт
HuH-7-CAS9 Cas9 20.01689911 3.60826302
HuH-7-CAS9 β-actin 16.40863609
HuH-7 Cas9 35.83909988 18.00432014
HuH-7 β-actin 17.83477974
Cutting Efficiency Validation
HuH-7-CAS9 cell line Product Validation Data（RT-QPCR）
Note: The above figure shows the sequencing peaks of the HuH-7-CAS9 stable cell pool which is electroporated by AGMAT gene targeting gRNA plasmid, after 48h antibiotic screening. The red arrow indicates the position where the nested peak appears, which shows that the genotype of the target site is significantly changed due to the cutting. Therefore, it indicates that Cas9 nuclease is successfully expressed.
STR Profile
Download STR Report
Loci STR Info (Sample Cell)
Sample Cell Line:HuH-7		 STR Info (Cell bank)
Cell Line:HuH-7
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 12121212
D13S317 10111011
D7S820 11111111
D16S539 10101010
VWA 16181618
TH01 7777
AMEL XXXX
TPOX 811811
CSF1PO 11111111
D12S391 2021
FGA 2223
D2S1338 1919
D21S11 3030
D18S51 1515
D8S1179 1414
D3S1358 1515
D6S1043 1315
PENTAE 1111
D19S433 1314
PENTAD 1212
D1S1656 1616
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Product Advantage

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    Easy to use
    Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations
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    High-efficient KO
    These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.
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    Curated cells
    Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.
Related Resources: Red Cotton Gene-editing System | ATCC | DSMZ | ECAZZ | Cellosaurus | CCTCC | NCBI
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